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Toxoplasma gondii ELISA can give false-positive IgM due to sphingolipids.
2003 OCT 2 - (NewsRx.com & NewsRx.net) -- Toxoplasma gondii ELISA can have unspecific IgM reactivity that corresponds with sphingolipids or ceramides found in pathogen, but not due to infection, in pregnant or grafted patients.
"During a routine serological survey, eight patients (five pregnant women, three grafted patients) were positive for Toxoplasma gondii-specific IgM by ELISA, but negative by a simultaneously performed immunosorbent agglutination assay," researchers in France report.
"No clinical or biological symptoms of toxoplasmosis were observed later, despite the absence of treatment. Only one IgM-reactive band, which corresponded to the low-molecular-weight antigen of Toxoplasma gondii, was observed by Western blot in these patient sera. Dot blotting of lipid extracts of Toxoplasma gondii demonstrated that this reactivity was directed against sphingolipids or ceramides," wrote A. Dao and coauthors.
The researchers concluded: "This IgM ...
- Toxoplasma gondii tachyzoite antigens elicit immune response in mice.
- Newspaper article from: Drug Week July 4, 2003 700+ words
...com & NewsRx.net) -- Toxoplasma gondii tachyzoite antigens elicited protective...excretory secretory antigens (ESA) of Toxoplasma gondii were evaluated in immunization of...immunized). The virulent RH strain of Toxoplasma gondii was used to challenge. Delayed...
- 3-D imaging of Toxoplasma gondii, host-cell interactions reported.
- Newspaper article from: Obesity, Fitness & Wellness Week December 25, 2004 700+ words
...visualize the interaction of the Toxoplasma gondii parasitophorous vacuole...The protozoan parasite Toxoplasma gondii is a representative of apicomplexan...dimensional imaging of Toxoplasma gondii - Host cell interactions...
- Toxoplasma gondii is present in the edible large rodent capybara.
- Newspaper article from: Immunotherapy Weekly November 19, 2003 700+ words
...com & NewsRx.net) -- Toxoplasma gondii is present in the edible large Brazilian...Brazil report. "Prevalence of anti-Toxoplasma gondii antibodies in 149 feral H. hidrochoeris...Parasitology (Seroprevalence of Toxoplasma gondii antibodies in the rodent capybara...
- Toxoplasma gondii 14-3-3 protein has 2 isoforms in cytoplasm, lipid rafts.
- Newspaper article from: Health & Medicine Week October 13, 2003 700+ words
...com & NewsRx.net) -- Toxoplasma gondii protein 14-3-3 has 2 isoforms...polyclonal antibody was raised against a Toxoplasma gondii 14-3-3-gluthatione S-transferase...localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid...
- Toxoplasma gondii infection inhibits lupus-like syndrome in autoimmune mice.
- Newspaper article from: Health & Medicine Week August 30, 2004 700+ words
...autoimmune disease demonstrated that Toxoplasma gondii infection can prevent development...mice, to study the effects of Toxoplasma gondii infection on the progress of lupus...findings in International Immunology (Toxoplasma gondii infection inhibits the development...
- Rare form is associated with Toxoplasma gondii infection.(pituitary...
- Newspaper article from: Cancer Weekly February 18, 2003 700+ words
...pituitary adenoma in association with Toxoplasma gondii infection. The two patients were...tumor cells, a finding confirmed by Toxoplasma gondii specific antibody immunohistochemistry...pituitary adenoma associated with Toxoplasma gondii infection. J Clin Pathol, 2002...
- Rare form is associated with Toxoplasma gondii infection.
- Newspaper article from: TB & Outbreaks Week February 18, 2003 700+ words
...pituitary adenoma in association with Toxoplasma gondii infection. The two patients were...tumor cells, a finding confirmed by Toxoplasma gondii specific antibody immunohistochemistry...pituitary adenoma associated with Toxoplasma gondii infection. J Clin Pathol, 2002...
- Vaccination protects B cell-deficient mice against Toxoplasma gondii.
- Newspaper article from: Medical Letter on the CDC & FDA December 19, 2004 700+ words
...oral challenge with mildly virulent Toxoplasma gondii. "Prior studies have demonstrated...highly virulent type I strain of Toxoplasma gondii parasites. However, it is not known...oral challenge with mildly virulent Toxoplasma gondii. Vaccine, 2004;22(29-30...
