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Autoschizis is the principal form of cell death from in vitro vitamins.
2003 SEP 4 - (NewsRx.com & NewsRx.net) -- Autoschizis is the principal form of cell death from in vitro vitamins.
According to a study from the United States, "Human ovarian carcinoma (MDAH 2774) cells were treated with sodium ascorbate (VC), menadione (VK3), or a combination of both in a ratio 100:1 for 1 h and then examined with scanning electron microscopy (SEM) and light microscopy (LM).
"Light microscopy data corroborated SEM observations, which demonstrated that death of VC + VK3-treated tumor cells occurred primarily by autoschizis. This type of cell death is characterized by a decrease in cell size, cytoplasmic self-excisions, and nuclear and nucleolar morphologic degradations without the formation of apoptotic bodies," wrote J. Gilloteaux and colleagues, M.E.I.O., Inc.
"Ultimately, cell death results from karyorrhexis and karyolysis. This study illustrates that plasma membrane damage (branching filopodia, blisters, blebs) results from VC treatment; cytoskeletal damage and self-morsellation are caused by VC, VK3, and VC+VK3 treatments," the researchers wrote.
"The VC treatment results in a 23% decrease in cell diameter while VK3-treated cells decrease cell diameter by 66%. After 1 h of VC+VK3 treatment, a heterogenous cell population is found. This population can be resolved into one population whose diameters are 23% smaller than those of sham-treated cells, and a second population whose diameters are ...
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Source: HighBeam Research, Autoschizis is the principal form of cell death from in vitro...