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Enzyme changes cause different abnormalities in oocytes and bone marrow cells.
2003 SEP 4 - (NewsRx.com & NewsRx.net) -- Enzyme changes cause different abnormalities in oocytes and bone marrow cells.
According to a study from the United States, "protein tyrosine phosphatases are needed for activating maturation promoting factor, meiotic spindle assembly and spindle checkpoint inactivation. The protein phosphatase inhibitor vanadate was used to upset the kinase-phosphatase equilibrium during oocyte maturation (OM) and the metaphase anaphase transition (MAT) prior to cytogenetic analyses of mouse oocytes and bone marrow cells. ICR females received pregnant mare serum gonadotrophin (PMSG) and 48 hours (h) later received human chorionic gonadotrophin (hCG)."
"Vanadate doses of 0, 5, 15, and 25 mg/kg were administered intraperitoneally immediately after hCG and ovulated oocytes and bone marrow cells were processed for cytogenetic analyses 18 h after hCG. Data were analyzed by Chi-square and Fisher's exact tests. Vanadate induced different cytogenetic abnormalities in oocytes and in bone marrow cells," stated J.B. Mailhes and colleagues, Louisiana State University, Health Sciences Center.
"The frequencies of oocytes exhibiting premature anaphase (spontaneous activation) in vanadate exposed mice were significantly (P
"These results suggest that alteration of ...
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Source: HighBeam Research, Enzyme changes cause different abnormalities in oocytes and bone...