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The gef gene blocks the growth of breast cancer cells.
2003 SEP 4 - (NewsRx.com & NewsRx.net) -- The gef gene blocks the growth of breast cancer cells.
According to recent research from Spain, "the gef gene has cell-killing functions in Escherichia coli. To evaluate the feasibility of using this gene as a new strategy for cancer therapy, we transfected it in MCF-7 cells derived from breast cancer (MCF-7TG). The gef gene was cloned in a pMAMneo vector under the control of a mouse mammary tumor virus promoter, inducible by dexamethasone (Dex), and was transfected with liposomes."
"After selection and induction, expression of the gef gene was confirmed by reverse transcription-polymerase chain reactions (RT-PCR) and Western blot. Cell viability was determined with a hemocytometer and the sulphorodamine B colorimetric assay, and the cell cycle was studied by propidium iodide (PI) staining," said H. Boulaiz and colleagues, University of Granada, School of Medicine.
"Annexin V-FITC and PI assays were used to evaluate apoptosis, which was confirmed by electron microscopy. In comparison with MCF-7 parental cells and MCF-7 cells transfected with an empty vector, MCF-7TG cells induced with Dex showed a significant decrease in proliferation rate, which was associated with evidence of ...
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Source: HighBeam Research, The gef gene blocks the growth of breast cancer cells.