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Recombinant influenza virus generated using baculovirus delivery vector.
2003 AUG 13 - (NewsRx.com & NewsRx.net) -- A recombinant influenza virus has been generated using a baculovirus delivery vector.
"A recombinant baculovirus vector containing mammalian cell-active promoters and transcription terminators was used to deliver a mutated influenza NS gene into Vero cells. In addition to the influenza NS gene, the baculovirus contained a reporter gene expression cassette (green fluorescent protein, GFP), allowing to monitor the Vero cell transduction efficiency," scientists in Austria and Thailand report.
"More than 90% of Vero cells were expressing GFP 24-48 hours post transduction," said Kanokwan Poomputsa and colleagues at the University of Natural Resources and Applied Life Sciences in Vienna and King Mongkut's University of Technology Thonburi in Bangkok. "After infecting baculovirus transduced cells with influenza helper virus, progeny of attenuated influenza virus carrying the recombinant NS gene could be selected. Baculovirus delivery was highly ...
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Source: HighBeam Research, Recombinant influenza virus generated using baculovirus delivery...