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Vector constructed from chimeric SIV and HIV characterized.
2003 AUG 13 - (NewsRx.com & NewsRx.net) -- A vpr vector constructed from chimeric simian and human immunodeficiency virus has been characterized.
"Chimeric simian and human immunodeficiency viruses (SHIVs) are useful toot for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector (designated as SHIV-3sj) by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication," investigators in Japan report.
"Five cytokine genes were inserted into SHIV-3sj and the ability of viral replication and expression of the inserted genes were examined," said Takeshi Haga and collaborators at Miyazaki and Kyoto Universities. "The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6, and IL-12p35 failed to become replication competent."
The researchers ...
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Source: HighBeam Research, Vector constructed from chimeric SIV and HIV characterized.