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The uptake of H-3-5HT by JAR cells occurs exclusively through SERT.
2003 JUL 3 - (NewsRx.com & NewsRx.net) -- The uptake of H-3-5HT by JAR cells occurs exclusively through SERT.
According to a study from Portugal, "The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP) in the JAR human choriocarcinoma cell line.
"As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of H-3-MPP+ and H-3-serotonin (H-3-5HT) was made," wrote F. Martel and colleagues, Faculty of Medicine, Department of Biochemistry.
"Specific uptake of H-3-MPP+ (0.2 microM) was temperature-, Na+- and potential-dependent. 5HT and MPP+ reduced H-3-MPP+ specific uptake (for 5HT, its IC50 was found to be 4 microM). The SERT inhibitors desipramine and fluoxetine also inhibited H-3-MPP+ specific uptake (with IC50S of 189 and 0.92 microM, respectively)," the researchers wrote.
"The inhibitors of the extraneuronal monoamine transporter (EMT) and of the organic cation transporter type 2 (OCT2), corticosterone and decynium22, had no effect on H-3-MPP+ specific uptake, but cyanine863 concentration-dependently reduced it (with an IC50 of 23 microM).
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Source: HighBeam Research, The uptake of H-3-5HT by JAR cells occurs exclusively through SERT.