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Needle-free delivery of DNA vaccine increases immune responses.
2003 JUL 2 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Needle-free intradermal injection of plasmid DNA-coated nanoparticles enhanced humoral and cellular immune responses in mice, according to a report in the Journal of Biotechnology.
"A high population of dendritic cells in the skin makes intradermal (ID) immunization an attractive route," said Zhengrong Cui and colleagues at the University of Kentucky. "We sought to further enhance immune responses from a previously reported novel nanoparticle-based DNA vaccine delivery system by administering the system intradermally into mouse skin using Biojector 2000, a needle-free jet injection device."
The investigators immunized BALB/c mice with 5 micrograms cytomegalovirus-beta-galactosidase (pDNA), nanoparticles coated with pDNA, or 10 micrograms beta-galactosidase protein plus 15 micrograms alum adjuvant using either a subcutaneous injection or the Biojector 2000. The mice received three vaccinations one week apart; at week 4 (day 28), the animals were sacrificed.
"For pDNA alone, jet and subcutaneous injection did not result in significant differences in IgG titer," said Cui and associates.
However, subcutaneous injection of the pDNA-coated nanoparticles stimulated a significantly stronger antigen-specific IgG response compared to immunization with the pDNA alone.
Delivery of the pDNA-coated nanoparticles using the Biojector 2000 elicited IgG antibody titers as much as 20-fold greater than did subcutaneous injections of the nanoparticles.
"In addition, jet injection of pDNA-coated nanoparticles enhanced the IgG titer by more than 200-fold over jet ...
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Source: HighBeam Research, Needle-free delivery of DNA vaccine increases immune responses.