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Plasmids encoding Japanese encephalitis virus proteins elicit immune response.
2003 MAY 21 - (NewsRx.com & NewsRx.net) -- Inoculation of plasmids encoding Japanese encephalitis virus PrM-E proteins with colloidal gold elicited a protective immune response in mice.
According to recent research published in the Journal of Virology, researchers in Japan have "established a simple and effective method for DNA immunization against Japanese encephalitis virus (JEV) infection with plasmids encoding the viral PrM and E proteins and colloidal gold. Inoculation of plasmids mixed with colloidal gold induced the production of specific anti-JEV antibodies and a protective response against JEV challenge in BALB/c mice."
"When we compared the efficacy of different inoculation routes, the intravenous and intradermal inoculation routes were found to elicit stronger and more sustained neutralizing immune responses than intramuscular or intraperitoneal injection," said Zijiang Zhao and associates at the Tokyo Metropolitan Institute for Neuroscience. "After being inoculated twice, mice were found to resist challenge with 100,000 times the 50% lethal dose (LD[subscript]50) of JEV (Beijing-1 strain) even when immunized with a relatively small dose of 0.5 microgram of plasmid DNA.
"Protective passive immunity was also observed in SCID mice following transfer of splenocytes or serum from plasmid DNA- and colloidal gold-immunized BALB/c mice. The SCID mice resisted ...
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Source: HighBeam Research, Plasmids encoding Japanese encephalitis virus proteins elicit immune...