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Sustained release of DNA achieved with lipid microtubules and agarose hydrogel.
2003 MAY 14 - (NewsRx.com & NewsRx.net) -- The sustained release of DNA was achieved with lipid microtubules and agarose hydrogel.
"Non-viral gene therapy typically results in low transfection efficiencies and transient gene expression. To address these limitations, two sustained delivery systems capable of releasing functional, compacted DNA for over 50 days were designed," investigators in the United States report.
"A luciferase plasmid was compacted with a polylysine-polyethylene glycol conjugate and released from agarose hydrogel and lipid microtubule-hydrogel delivery systems for over 50 days," said Nancy J. Meilander and collaborators at Case Western Reserve University and Copernicus Therapeutics, Inc. "The released DNA was characterized structurally using sedimentation, electron microscopy, and serum stability, and functionally using in vitro transfections.
"The released DNA retained its physical compaction and nuclease resistance and was converted from supercoiled to nicked and linear forms. Released compacted DNA produced significant gene expression ...
- New microscopy study findings have been reported by scientists at University of...
- Newspaper article from: Science Letter November 10, 2009 700+ words
"A simplified approach to increase neuronal attachment on an agarose hydrogel was proposed by blending agarose with another biocompatible polysaccharide, chitosan. The stiffness of the agarose gel was maintained...
- Recent studies from Columbia University add new data to life sciences.
- Newspaper article from: Blood Weekly October 29, 2009 700+ words
...cartilage." Ng and colleagues published their study in Tissue Engineering Part a (Zonal Chondrocytes Seeded in a Layered Agarose Hydrogel Create Engineered Cartilage with Depth-Dependent Cellular and Mechanical Inhomogeneity. Tissue Engineering Part a...
- Data on engineered cartilage discussed by researchers at National Institutes of...
- Newspaper article from: Blood Weekly January 15, 2009 700+ words
...transient growth factor supplementation to promote robust maturation of tissue-engineered cartilage." "Bovine chondrocyte agarose hydrogel constructs were cultured under free-swelling conditions in serum-containing or SF medium supplemented continuously...
- The effects of crosslinker and monomer to the polymerization of...
- Magazine article from: American Journal of Applied Sciences Saion, Elias Shaari, A. Halim Rahman, M. Zaki A. Zaman, M.D. Khairul Kadni, Taiman Doyan, Aris Susilawati August 1, 2005 700+ words
...acrylamide (AA, monomer) and N,N' methyelene-bis-acrylamide (BIS, crosslinker) [3] dissolved in a gelatin/agarose hydrogel. Upon irradiation, water molecules dissociate into OH and H radicals that break the double C=C bonds of monomers...
- Research from Columbia University reveals new findings on biochemistry.
- Newspaper article from: Blood Weekly March 19, 2009 700+ words
...Osteoarthritis and Cartilage (Analysis of radial variations in material properties and matrix composition of chondrocyte-seeded agarose hydrogel constructs. Osteoarthritis and Cartilage, 2009;17(1):73-82). For additional information, contact C.T...
- Researchers from Columbia University, Department of Biomedical Engineering...
- Newspaper article from: Blood Weekly November 6, 2008 700+ words
...as a base material for the tissue-engineering of osteochondral constructs for cartilage repair. Chondrocyte-seeded agarose hydrogel constructs were cultured alone or attached to an underlying bony base in a chemically defined medium formulation that has...
- Research from Columbia University, Department of Biomedical Engineering in the...
- Newspaper article from: Blood Weekly January 1, 2009 700+ words
...mechanism that has only been predicted recently from theory, but not yet observed experimentally." "This study uses agarose hydrogel and dextran molecules as a model experimental system to explore this mechanism. Results show that dynamic loading can...