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Heat-directed suicide gene therapy reduces breast cancer cell survival.
2003 MAY 8 - (NewsRx.com & NewsRx.net) -- by Sonia Nichols, senior medical writer-Scientists in Ontario, Canada have discovered that heat-directed suicide gene therapy can reduce breast cancer cell survival.
Researchers at the University of Toronto and Ontario Cancer Institute believe that treatment strategies combining gene therapy with heat could prove beneficial for overcoming the heat or radiation resistance of certain breast cancers.
"Previously, we demonstrated that infection of human breast cancer cells with a recombinant adenovirus expressing beta-galactosidase from the human heat shock protein (hsp)70b gene promoter (Ad.70b.betagal) results in 50- to 800-fold increases in reporter gene expression following heat treatment (30 minutes at 43 degrees C)," said Anthony M. Brade of the University of Toronto.
Building on that study, Brade and colleagues incorporated an Escherichia coli cytosine deaminase/herpes simplex virus thymidine kinase (CDTK) fusion gene into constructs that already contained the hsp promoter. The fusion gene causes cells to self-destruct in the presence of specific prodrugs.
Researchers infected two breast cancer cell lines (T47D and MCF-7) with the heat-directed suicide gene therapy Ad.70b.CDTK. When cells were subsequently treated with 30 minutes of mild heat and fluorocytosine and ganciclovir, clonogenic survival fell from 30- to 60-fold as compared to cells treated with either ...
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- Newspaper article from: Cancer Gene Therapy Week October 4, 2004 700+ words
...amp; NewsRx.net) -- Suicide gene therapy containing recombinant viral...to systemic chemotherapy. Suicide gene therapy may be an alternative treatment...development of rAAV-2-based suicide gene therapy of sarcomas," they predicted...
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- Newspaper article from: Cancer Gene Therapy Week August 23, 2004 700+ words
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- Suicide gene therapy, ganciclovir, and irradiation produce cancer regression.
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...NewsRx.com & NewsRx.net) -- Suicide gene therapy, ganciclovir, and irradiation produce cancer...Kanazawa and colleagues published their study in Gene Therapy (Suicide gene therapy using AAV-HS Vtk/ganciclovir in combination...
- Suicide Gene Therapy Cell-Specific Transgene Expression Prevents HSVtk/GCV...
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- Suicide Gene Therapy "Suicide Gene Therapy of Ovarian Cancer: An Experimental...
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- Liposomal suicide gene therapy attacks peritoneal cancer.
- Newspaper article from: Cancer Gene Therapy Week February 16, 2004 700+ words
...com & NewsRx.net) -- Liposomal suicide gene therapy attacks peritoneal cancer. "Peritoneal...colleagues published their study in Cancer Gene Therapy (Combined suicide gene therapy for pancreatic peritoneal carcinomatosis...
- Review supports suicide gene therapy approach.
- Newspaper article from: Gene Therapy Weekly October 10, 2002 700+ words
...Medicine researchers supports suicide gene therapy as a strategy for efficient...normal host cells acceptable. Suicide gene therapy strives to deliver genes...identified articles related to suicide gene therapy for cancer. "A number of...
- Cancer Gene Therapy Connexin 43 Affects Suicide Gene Therapy Bystander Effect.
- Newspaper article from: Gene Therapy Weekly October 19, 1998 700+ words
...thymidine kinase (HSVtk)/ganciclovir (GCV) suicide gene therapy. Recent research has suggested that Cx43 may influence...to influence the bystander effect of HSVtk/GCV suicide gene therapy (F.D. Vrionis et al., Gene Therapy 1997...