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Transdermal gene delivery eradicates tumors expressing hepatitis C proteins.
2003 MAY 7 - (NewsRx.com & NewsRx.net) -- by Sonia Nichols, senior medical writer-A series of research experiments has demonstrated that hepatitis C virus (HCV) DNA vaccine can suppress tumor growth.
The vaccine, developed by researchers at the Karolinska Institute at Huddinge University Hospital in Stockholm, Sweden, contains the genetic components of HCV nonstructural proteins. The gene gun method of delivery appears particularly suited for its administration, researchers report.
"The HCV protease and helicase encompasses the nonstructural (NS)3 protein and the cofactor NS4A, which targets the NS3/4A complex to intracellular membranes," described L. Frelin and coauthors.
Study authors developed expression vectors containing NS3/4A or NS3 only. NS4A augmented the expression of NS3 after transfection, they noted.
"Subsequently, immunization with the NS3/4A gene primed 10- to 100-fold higher levels of NS3-specific antibodies as compared to immunization with the NS3 gene," Frelin's group reported.
The NS3/4A gene also generated an immunoglobulin (Ig)G2a/IgG1 ratio that was more than six times higher than that generated by the NS3 gene. These findings signaled a strong humoral response.
Low dose NS3/4A gene vaccine, delivered intramuscularly, suppressed the ...
- Gene Gun-Mediated Vaccine Induces Antitumor Immunity.
- Newspaper article from: Immunotherapy Weekly February 27, 2000 700+ words
...com) -- A gene gun-mediated DNA...Johns Hopkins University Hospital, Maryland...Gene Therapy ("Gene Gun-Mediated DNA...researchers used the gene gun method to vaccinate...Johns Hopkins University Hospital, Department...
- Gene Gun-Mediated Vaccine Induces Antitumor Immunity.(Brief...
- Newspaper article from: Vaccine Weekly February 23, 2000 700+ words
...com) -- A gene gun-mediated DNA...Johns Hopkins University Hospital, Maryland...Gene Therapy ("Gene Gun-Mediated DNA...researchers used the gene gun method to vaccinate...Johns Hopkins University Hospital, Department...
- p21Waf1 gene transfer via gene gun technology limits esophageal cancer growth.
- Newspaper article from: Proteomics Weekly December 6, 2004 700+ words
...net) -- p21Waf1 gene transfer via gene gun technology limits esophageal cancer growth...therapy in esophageal cancer, we used gene gun technology, which proved to be a powerful...of p21Waf1 protein after applying this gene gun technique," said Y. Tanaka and colleagues...
- Scientists develop new gene gun to assist in fight against cancer
- Newspaper article from: The Boston Globe Richard Saltus, Globe Staff March 20, 1995 700+ words
...weapon in the fight against cancer -- a "gene gun" that shoots pure DNA directly into tumors. The gene gun can insert genes into cells in seconds...these are the first experiments using a gene gun in tumor-bearing animals," said Wenn...
- Gene Gun Immunization using Plasmid DNA Expressing IFN-(alpha) or IL-12 in...
- Newspaper article from: Immunotherapy Weekly June 29, 1998 700+ words
"Gene Gun Immunization using Plasmid DNA Expressing...following particle bombardment of the skin ('gene gun' immunization) in comparison with those...T helper cell responses. However, gene gun immunization predominantly promotes the...
- 'GENE GUN' SCORES GENETIC BULL'S-EYE.(Living Today)
- Newspaper article from: Albany Times Union (Albany, NY) June 5, 1990 700+ words
...Michael Kelly Newhouse News Service A "gene gun" invented by Cornell University researchers...for the two companies used Cornell's "gene gun" to fire microscopic "bullets" coated...the improved crop varieties made by the gene gun on feed store shelves for several more...
- Gene gun-based transfection examines the underpinnings of melanogenesis.
- Newspaper article from: Biotech Week September 1, 2004 700+ words
...NewsRx.com & NewsRx.net) -- Gene gun technology transfectiion examines human...signaling protein cDNA. "We developed a gene gun method for the transfer of human agouti...delivered to the skin of Long Evans rats by gene gun bombardment. Skin pigmentation, body...
- Gene Gun Generates Effector T Cells for Adoptive Therapy.(Brief Article)
- Newspaper article from: Gene Therapy Weekly April 6, 2000 700+ words
...APR 6 - (NewsRx.com) -- Using the gene gun for plasmid bombardment induces generation of effector T cells. "We utilized the gene gun to transfect subcutaneous D5 melanoma...journal Cancer Immunology Immunotherapy ("Gene gun application in the generation of effector...
