AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Amino acid residues confer temperature sensitivity to influenza virus strains.
2003 MAY 7 - (NewsRx.com & NewsRx.net) -- Multiple amino acid residues conferred temperature sensitivity to human influenza virus vaccine strains (FluMist) derived from cold-adapted A/Ann Arbor/6/60.
"FluMist influenza A vaccine strains contain the PB1, PB2, PA, NP, M, and NS gene segments of ca A/AA/6/60, the master donor virus-A strain. These gene segments impart the characteristic cold-adapted (ca), attenuated (att), and temperature-sensitive (ts) phenotypes to the vaccine strains. A plasmid-based reverse genetics system was used to create a series of recombinant hybrids between the isogenic non-ts wt A/Ann Arbor/6/60 and MDV-A strains to characterize the genetic basis of the ts phenotype, a critical, genetically stable, biological trait that contributes to the attenuation and safety of FluMist vaccines," scientists in the United States report.
"PB1, PB2, and NP derived from MDV-A each expressed determinants of temperature sensitivity and the combination of all three gene segments was synergistic, resulting in expression of the characteristic MDV-A ts phenotype," stated Hong Jin and colleagues at MedImmune Vaccines, Inc. in San Diego. "Site-directed mutagenesis analysis mapped the MDV-A ts phenotype to the ...
- Changes in temperature sensitivity of development form sowing to flowering in...
- Magazine article from: Crop Science Yin, Xinyou Kropff, Martin J. Goudriaan, Jan November 1, 1997 700+ words
...different crops have shown that model parameters for temperature sensitivity differed among developmental phases (Robertson, 1968...have suggested, in one way or another, changes in temperature sensitivity or the base and optimum temperatures throughout preflowering...
- Seasonal variations in soil respiration and temperature sensitivity under three...
- Magazine article from: Australian Journal of Soil Research Wang, XiaoGuo Zhu, Bo Gao, MeiRong Wang, YanQiang Zheng, XunHua December 1, 2008 700+ words
...and climatic zones (Raich and Schlesinger 1992). Temperature sensitivity of soil respiration ([Q.sub.10]) has been widely...temperature and soil moisture affecting the variation of temperature sensitivity on similar soils under the same climate conditions...
- Thermal imagers with 0.08[degrees]C temperature sensitivity.(Leading Off)
- Magazine article from: ECN-Electronic Component News August 1, 2006 700+ words
The Fluke T140 and T145 FlexCam[R] 160 X 120 thermal imagers with temperature sensitivity of 0.08[degrees]C are designed to deliver high resolution images that capture small temperature differences. The Windows...
- Research on Escherichia coli reported by scientists at Okayama University.
- Newspaper article from: Biotech Week June 24, 2009 700+ words
...enhance the temperature-sensitive (ts) phenotype of an E. coli strain defective in the...periplasmic proteases." "This severe ts phenotype was relieved only partially by an SsrA...chaperone activity, could relieve the ts phenotype of the double mutant, suggesting that...
- Genetic analysis of influenza virus master donor strain reported.
- Newspaper article from: Medical Letter on the CDC & FDA July 18, 2004 700+ words
...sequencing in order to understand the variability in the ts phenotype." "This study has also addressed the feasibility of using...retaining the defined mutations that contribute toward the ca/ts phenotype," concluded the investigators. Youil and associates published...
- New influenza research has been reported by scientists at University of...
- Newspaper article from: Virus Weekly March 3, 2009 700+ words
...assigning the ts lesion to segment 5. Introduction of this mutation into A/PR/8/34 virus by reverse genetics produced a ts phenotype, confirming the identity of the lesion. Despite an approximately 100-fold drop in the viral titer at the nonpermissive...
- New findings reported from R. Mauritz and co-authors describe advances in colon...
- Newspaper article from: Cancer Weekly September 1, 2009 700+ words
...fluoropyrimidines," wrote R. Mauritz and colleagues. The researchers concluded: "However, the relationship between TS phenotype and TSER genotype in normal tissues warrants further investigations in large-scale prospective studies evaluating TS genotype...
- Parainfluenza virus polymerase mutations provide varying attenuation.
- Newspaper article from: Medical Letter on the CDC & FDA April 25, 2004 700+ words
...the L polymerase of recombinant human parainfluenza virus type 1 (rHPIV1). In rHPIV1, the Y942H mutation specified the ts phenotype in vitro and the attenuation (att) phenotype in hamsters, whereas the L992F mutation specified neither phenotype...
Source: HighBeam Research, Amino acid residues confer temperature sensitivity to influenza virus...