Bioscience Technology articles from October 2006

Bioscience Technology archives from October 2006

Breaking through the proteomic bottleneck.(Cover story)
October 1, 2006... More than 300 years ago, Anton van Leeuwenhoek discovered the secrets of building compound microscopes, and biologists have been obsessed with the minuscule ever since. It's an understandable fascination; from Streptococcus to sarcoma to...

Economic conditions for your organization.(SNAPSHOT)
October 1, 2006... SNAPSHOT Economic conditions for your organization % Respondents Positive (growth) 54.6 Very Positive (Significant Growth) 17.7 No Real Change (Growth) 17.7 Negative (Decline) ...

Do you have a best practice?(Editor's Note)(Editorial)
October 1, 2006... This past June "Bioscience Technology began a new section called Best Practices. This publication's mission has always bean about one thing--the latest chemicals, instruments, apparatus and labware and how they are used to advance biological...

In vivo imaging system.(October's Innovations)
October 1, 2006... Xenogen IVIS Spectrum is an in vivo optical system that uses both bioluminescent and fluorescent imaging to "light up" biological processes, including tumor detection. The systems gives researchers more imaging options and opens up the...

Protein refolding system.(October's Innovations)
October 1, 2006... PreEMT E-150 System rapidly and effectively solubilizes and refolds proteins from aggregated protein samples. The system provides a high success rate for solubilizing and refolding difficult or intransigent proteins. Up to 28 1-ml samples under...

Baculovirus selection kit.(October's Innovations)
October 1, 2006... BacMagic DNA provides positive selection of baculovirus recombinants for protein expression in insect cells, eliminating plaque purification steps. It is an AcNPV genome with a deletion in the essential open reading frame (ORF) 1629 and an...

Disposable lab-on-a-chip system.(October's Innovations)
October 1, 2006... The PHOTONICLAB Platform combines printed organic semiconductor based optoelectronics with microfluidics to create a disposable lab-on-a-chip that enables mobile, real-time and multi-parameter detection of chemical and biological substances....

High-throughput label-free drug screening.(October's Innovations)
October 1, 2006... Epic high-throughput label-free screening platform uses changes in light wave reflections to detect interactions between a library of chemical compounds and a therapeutic target or diseased cells. The system eliminates the need to manually...

Knockout/knock-in mice.(October's Innovations)
October 1, 2006... Hybrid 129xC57 embryonic stem (ES) cell line exhibits a high rate of homologous recombination in the production of mouse knockout and knock-in models. These characteristics have resulted in reduced time for electroporation and screening tasks,...

Single cell isolation system.(October's Innovations)
October 1, 2006... The CytoClone system applies phase contrast and fluorescence imaging technology to the isolation and analysis of pure cell clones. Generating high resolution cell imagery in real time, it allows users to select target cells using morphologic of...

Miniblotters process westerns.(October's Innovations)
October 1, 2006... Miniblotters enable users to incubate different antibodies with antigens run in separate gel lanes and transferred to a membrane. In practice, an intact blotted membrane is mounted directly in the unit and the immunoassay is run in the...

shRNA constructs for gene specific knock down.(RNA Focus)
October 1, 2006... HuSH-29 shRNA constructs for gene specific knock down provide long, 29-mer duplexes, which offer high potency, less off-target effect and reduced interferon induction. The product line covers every human gene locus. Four constructs are provided...

Extract RNA from fresh or frozen tissue.(RNA Focus)
October 1, 2006... Agencourt RNAdvance Tissue extraction technology combines with a Beckman Coulter automation system to offer a walk-away platform for recovery of high quality total RNA in a multiwell format. Using Agencourt's SPRI (Solid Phase Reversible...

siRNA libraries.(RNA Focus)
October 1, 2006... New versions of Ambion's Silencer siRNA Libraries for human kinases, phosphatases, the drug-gable genome, the extended druggable genome, and the human genome feature updated collections of siRNAs in a convenient format. Three individual siRNAs...

siRNA transfection reagent.(RNA Focus)
October 1, 2006... RiboJuice siRNA transfection reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. The optimized, easy-to-use reagent is formulated for minimal toxicity. RiboJuice and GeneJuice, a transfection reagent...

PCR-ready DNA from FFPE tissue in 30 minutes.(Biochemicals & Kits: Application Note)
October 1, 2006... Introduction A commonly used method for tissue preservation has been formalin-fixation followed by paraffin-embedding (FFPE). In recent years there has emerged the desire for molecular information from these archival tissues. One notable...

Fluorescence dyes.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... PromoFluor dyes span the wavelength spectrum from blue-green to far red. They provide photostability, strong absorption, high fluorescence quantum yield and water solubility. Dye intensity increases when coupled to biomolecules resulting in...

ShRNA Human Kinase Gene Family.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... MISSION TRC shRNA Human Kinases Gene Family, the second in a series of shRNA target sets, allows rapid RNAi cell-based screens in mammalian systems not easily manipulated by traditional siRNA. The lentiviral transduction particle format...

Labeled phosphoinositides.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... GloPIPs, phosphoinositides labeled with BODIPY-FL, BODIPY-TMR or biotin, are available in aqueous and lipophilic configurations. Phosphoinositides are found in cellular membranes and playa vital role as second messengers for diverse cellular...

Normalized cancer tissue cDNAs.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... TissueScan is a normalized panel of cDNAs from cancer tissues at various stages of progression. Scientists can obtain a gene expression profile or a SNP profile across 200 cancer tissues using simple, real-time PCR with gene-specific or...

Reagents isolate human regulatory T cells.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... These reagents detect human regulatory T (Treg) cells, a cell involved in cell and tissue transplantation, cancer immunotherapy and autoimmune disease research. A three-color surface staining approach for flow cytometric analysis of CD4, CD25...

Human IgG Kit.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... Used in screening for high producing clones or quantifying antibody levels for production, the Guava RapidQuant Human IgG Kit offers efficient antibody quantitation: no washing steps, no dilution of typical hybridoma supernatants, no...

Human Papilloma Virus (HPV) antibodies.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... Research studies have shown Human Papilloma Virus (HPV) is present in most cervical cancer. Some recent research studies have shown that HPV testing may be more sensitive than traditional Pap smears at detecting precancerous lesions of the...

Rapid Ligation Kit.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... Ligate-IT Rapid Ligation Kit provides high-quality reagents for fast and easy ligation reactions. Ligation reactions are complete in five minutes for cohesive ends and 10 minutes for blunt ends. It includes USB T4 DNA Ligase, Nuclease-Free...

Blot enhancing solution.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... Western Blaster solution increases sensitivity when Alkaline Phosphatase and BCIP/NBT are used to develop the Western blots. Duration of incubation of primary antibody is reduced. Signals appear within 5 minutes. The solution is provided in 25...

HTRF assays for cGMP, PGE2 and cortisol.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... Related to important targets in the fields of inflammation and metabolic diseases, these assays for cGMP, PGE2, and cortisol integrate proprietary d2 acceptor dye, optimizing assay robustness, performance, and stability. The first assay is...

Gene expression assays.(Biochemicals & Kits: PRODUCTS)
October 1, 2006... For assays that detect gene expression levels, validate microarray data and verify knockdown, SYBR GreenER qPCR reagents feature a double-stranded DNA binding dye with an excitation/emission spectrum nearly identical to SYBR Green dye. Bright...

Copy Number analysis software.(Bioinformatics Focus)
October 1, 2006... ArrayAssist Copy Number Software (Stratagene) is a guided workflow system that enables researchers to manipulate and analyze the massive data sets generated by chromosomal copy number microarrays. The Copy Number package is Stratagene's most...

Data repository analysis software.(Bioinformatics Focus)
October 1, 2006... Benchware Discovery 360 software provides a single point of entry from which researchers can access, analyze and share data from disparate enterprise biological and chemical repositories. It integrates seamlessly with applications from other...

Informatics system for metabolomics research.(Bioinformatics Focus)
October 1, 2006... Version 7.5 of the KnowItAll Informatics System--the Metabolomics Edition--integrates Infometrix's Pirouette chemometrics technology with the KnowItAll platform, databases and Nuclear Magnetic Resonance tools. This end-to-end metabolomics...

Sequence analysis software.(Bioinformatics Focus)
October 1, 2006... Lasergene 7.1 (DNASTAR, Inc., Madison, WI) expands the capabilities of this sequence analysis software by introducing SeqMan Pro, an assembly engine built to assemble large projects (up to 1 million sequences) and also built to work well with...

Mass spectrometer.(Instruments & Systems: PRODUCTS)
October 1, 2006... Mass resolution and accuracy, sensitivity, analytical versatility and reliability have been enhanced in the new autoflex III smartbeam mass spectrometers for use in expression proteomics and quantitative proteomics, as well as in...

Optimized chemogenetic kinase arrays.(Instruments & Systems: PRODUCTS)
October 1, 2006... Peakfinder chemogenetic arrays are focused collections of drug-like molecules targeted towards specific receptor families and pre-optimized for robustness and bioavailability in man. They are designed to enhance receptor-ligand interactions...

Efficient multiplex PCR optimization.(Instruments & Systems: Application Note)
October 1, 2006... Introduction Multiplex PCR is a complex reaction that often results in low product yield, product drop out and nonspecific product amplification. (1) This is due to the different optimal annealing temperatures and competition of the...

Atomic force microscope.(Instruments & Systems: PRODUCTS)
October 1, 2006... NanoMan II atomic force microscope features a Hybrid XYZ scanner operates in closed-loop mode in all three axes, enabling nanomanipulation and life science imaging. Closed-loop feedback with the XYZ head provides precise X/Y control for...

DNA production service.(Instruments & Systems: PRODUCTS)
October 1, 2006... The TriathlonT system enables the mass production of specific DNA sequences via polymerase chain reaction. Milligram and gram quantities of custom PCR-amplified DNA are available for a variety of applications, such as diagnostic standards,...

Flow chemistry system.(Instruments & Systems: PRODUCTS)
October 1, 2006... FRX systems consist of a number of separate modules that can be connected together for conducting various flow chemistry experiments. Reactions occur under controllable, laminar flow conditions. The systems are modular, allowing selection of...

NMR Metabolic Profiling Database.(Instruments & Systems: PRODUCTS)
October 1, 2006... NMR Metabolic Profiling Database, for metabolite identification and quantification, contains information on the most common endogenous metabolites in body fluids, as well as on known metabolites of some over-the-counter drugs and selected food...

TIRF System.(Instruments & Systems: PRODUCTS)
October 1, 2006... AM TIRF System combines fluorescence imaging, sensitivity, and an optimum signal-to-noise ratio to ensure accurate results for examinations of close-to-membrane structures. The system features a large TIRF field of view, complete control of the...

Protein purification kits.(Instruments & Systems: PRODUCTS)
October 1, 2006... ProPur Kits purify proteins from initial fractionation to final polishing steps in yields typically associated with a gravity drip column, but with the speed of a spin column. IgG, IgA and His-tagged proteins can be purified in 20 to 50...

Automated Real-time QPCR.(Instruments & Systems: PRODUCTS)
October 1, 2006... Automation and Real-time qPCR Systems combine accurate pipetting with sensitive real-time cycling. By combining the fast temperature ramping, short detection times and intuitive assay programming of the Mastercycler ep realplex with the...

Resequencing software.(Instruments & Systems: PRODUCTS)
October 1, 2006... TargetSeq Resequencing System (Applied Biosystems, Inc.) features a fast-run module for the sequencing of individual gene exons or other small DNA fragments. It is able to produce 200 to 400 base pair sequencing reads in 20 minutes or less,...

Prep column guard cartridge.(Instruments & Systems: PRODUCTS)
October 1, 2006... Iso-Prep Guard offers column protection for adsorptive samples and a proven sample distribution mechanism via a precision machined holder. Its short 10 mm length yields low pressure drop at flow rates in excess of 50 ml/min. It is ideal for...

UV/Vis/NIR spectrophotometer.(Instruments & Systems: PRODUCTS)
October 1, 2006... V-600 Series UV/Vis/NIR spectrophotometers are compact and provide high speed scanning. System features include the "IQ Accessory" function for automatic accessory recognition and the "IQ Start" function for immediate start when conducting...

Identify lead compounds.(Instruments & Systems: PRODUCTS)
October 1, 2006... Hit Profiler analysis module identifies lead compounds from high throughput screening results. Semantic integration technology is exploited to display screening results and a diverse range of information in a compound-centric, interactive...

Chemical inventory software.(Instruments & Systems: PRODUCTS)
October 1, 2006... NEXXIS Chemical Inventory Manager (CIM) v2.1 enhances scaling for enterprise environments. Integration methods allow seamless interaction with specific applications and standard NEXXIS application modules like qELN, Sample Tracker and...

Compact bioreactor.(Filtration Focus)
October 1, 2006... BIOSTAT A plus compact bioreactor systems can be autoclaved. Standard notebook-pc interface delivered with the unit enables operation of the system and transfer of measured data to an installed MFCS/DA SCADA software package for further...

Conductivity/temperature flow cells.(Filtration Focus)
October 1, 2006... Although designed for single-use bag manifold applications, SciLog flow cells withstand many test/cleaning cycles and, if required, can be easily re-calibrated. The pre-sterilized flow cells offer stored sensor ID and calibration data....

Sealed cell culture system.(Filtration Focus)
October 1, 2006... OptiCell microplate-sized culture chamber provides a growth surface of 100 [cm.sup.2]. It takes up a fraction of the space and half the media volume of 75 [cm.sup.2] flasks. Cells grow on clear, non-fluorescent polystyrene membrane surfaces...

Filter cups sterilize media, buffers.(Filtration Focus)
October 1, 2006... Stericup Filter Cups are fast-flowing, low protein-binding cups for sterilizing tissue culture media buffers and other aqueous solutions. A new ergonomic design incorporates recessed finger grips on the receiver flask that make it easier to...

HPLC pump.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Model 584 HPLC Pump provides pulse-free solvent delivery even at flow rate ranges below 50 [micro]l/min. Flow rate range of 0.0001 to 10 [micro]l/min makes the pump useful in analytical, microbore and LC-MS separations. Using dual parallel...

Robotically-controlled vacuum nest.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... VaryVac, a robotically-controlled vacuum nest for filter plates used in DNA prep, protein purification, solid-phase separations and other microplate-based filtration, combines automated lid opening and sealing with closed-loop control of...

Bar code readers.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Bar code readers SmartScan 96, for reading tubes in 48 and 96 place racks, and the SmartScan Solo single tube reader (ABgene Inc., Rochester, NY), are offered for use with ABgene's SmartScan 2D coded cluster tubes. The instruments rapidly...

Interchangeable digital camera systems.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... The DS-Fil digital camera head and DS-L2 and DS-U2 control units have been added to Nikon's Digital Sight Series. The camera head improves resolution, expands dynamic range and reduces noise, while the control units provide improved platforms...

Fluorescence filter sets.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... BrightLine Clinical hard-coated fluorescence filter sets feature durable, research-grade BrightLine filters. The "no burn-out" feature lowers the total cost of ownership and ensures medical and clinical users of reproducible measurements year...

Contact rack plate.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Scienceware Contact Plate Rack can reduce handling accidents during transport and incubation of critical contact plates. Each rack holds up to 60 contact plates with lids in a stable configuration protected from knock-over and lid-displacement....

Mill for high-speed sample processing.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Utilizing a high-torque motor and cassette principle, the Ultra Centrifugal Mill ZM 200 can handle hundreds of samples per day without cross contamination. The mill is versatile, safe and easy to use. Size reduction takes place by impact and...

Dual syringe pump.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Dual Syringe Pump 33 provides two independent pumping channels linked through hardware and software. Numerous modes of operation are available. Reciprocal/Parallel Mode allows the two pumping mechanisms to run completely independently in the...

Cell culture flasks and chambers.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Flasks in 25 and 75 [cm.sup.2] volumes and CellSTACK Culture Chambers offer an ultra-low attachment surface and are well-suited for applications including stem cell, cancer, and immune system research. The scale of cell culture can be...

Online cell monitoring, analysis.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Bionas 2500 benchtop instrument simultaneously measures multiple metabolic parameters, including acidification, oxygen consumption and adhesion, in living cells, facilitating predictive pharmacology safety studies during preclinical phases of...

Data acquisition processor.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... The DAP 5216a/627 includes 16 analog inputs, 2 analog outputs, 16 digital inputs, and 16 digital outputs. External rack-mounted hardware can extend these channel counts to 512, 66, 128, and 1024 respectively. The board can acquire 16-bit data...

Temperature data recorder.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Cryo-Temp data recorder can be placed in environments ranging in temperature from -86 C to + 35 C. Offered for use in dry ice shipment and storage monitoring, blood banks and laboratory freezers, the battery operated, stand-alone data logger...

Bilayer clamp amplifier.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... The BC-535 Bilayer Clamp Amplifier records single channel currents from planar lipid bilayers. The amplifier features an extremely low noise, resistive feedback head stage capable of measuring currents from sub-pA to 20 nA. Additional features...

Bioscience spell checking.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Bioscience spelling software for 2006 includes over 200,000 specialty words. It saves time and increases accuracy, providing correct spelling choices for incorrectly spelled words from within the many biological disciplines. Users can look up...

Laboratory hoods.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Bench-top Protector Pass-Through Hood can be operated from either side. In island or peninsular configurations, unobstructed viewing from either side makes the hood ideal for chemistry demonstrations or observing students. An optional, 15" x...

Micro annular gear pumps.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Micro annular gear pumps are offered in Low Pressure (Series ML) or High Performance (Series MH) configurations. Precision rotors provide tight flow rate control, even at differential pressures as high as 80 bar (1,160 psi). These rotors allow...

Water-bath incubation system.(Apparatus & Consumables: PRODUCTS)
October 1, 2006... Water-bath Insert for the SciGene Corp. Hybex Incubator eliminates the need for hot plates or water baths to heat water or buffer solutions. The insert is useful for washing DNA microarrays or for slides used in FISH/ISH. It is designed for...

Reverse capture autoantibody array probes for prostate-cancer proteins.(Best Practices)
October 1, 2006... Oncologists need a new test for prostate cancer--the most common form of cancer diagnosed in American males over the age of 50. Any male tested for prostate cancer, though, knows that an oncologist can measure the level of prostate-specific...