Bioscience Technology articles from August 2006

Bioscience Technology archives from August 2006

Microfluidics: small volumes, mind-bending speed.
August 1, 2006... The clock ticks forward one second. A technician pipets a sample from a crude cellular extract, processes it through a complex preparation protocol with multiple reagents, runs the processed extract on a gel, isolates the desired bands, and...

Fractionate proteins, peptides, run multiple parallel samples, produce fractions in solution.
August 1, 2006... Agilent's 3100 OFFGEL Fractionator fractionates intact proteins and peptides by their isoelectric point (pI). It offers resolution up to 0.1 pH units, analytical-to-preparative sample loading capacity and the ability to run multiple samples in...

Top five research challenges.(SNAPSHOT)
August 1, 2006... Top five research challenges Shortening the time required for research 63.3 Increased pressure to reduce costs 58.3 Increased pressure for overall productivity 55.5 Obtaining grants ...

Scientists are rock stars.(Editor's Note)
August 1, 2006... Do you know Morgan Spurlock? He was the auteur/subject of Super Size Me, the 2004 documentary covering what happens to someone who eats exclusively at McDonald's for a month. I have not seen the movie, but I understand it was not pretty. ...

Live Cell Array.(August's Innovations)
August 1, 2006... The NUNC LiveCell Array, a microscope slide-based high content analysis (HCA) tool, enables multi-parametric imaging-based assays on thousands of intact individual cells, including non-adhering blood and bone marrow cells. Cells can be observed...

Image analysis software.(August's Innovations)
August 1, 2006... New version of VisiQuest software features a toolbox with 60 additional functions for image registration and segmentation tasks. Designed to save time and effort by eliminating manual steps in image registration, the new toolbox provides a...

Optimize liquid handling.(August's Innovations)
August 1, 2006... MVS ADVANCED liquid delivery verification system is used with non-aqueous test solutions to verify multichannel instrument performance. Used on the bench, the portable measurement system provide volumetric measurements in minutes, and is...

Triple stack incubator/shaker.(August's Innovations)
August 1, 2006... I26 and I26R shakers can be stacked up to three units high, with independent control for each shaker. Offering speeds of 25 to 400 rpm and temperatures from 7 to 60 C (from -15 C in the refrigerated I26R), the shakers culture an array of...

Biological safety cabinet.(August's Innovations)
August 1, 2006... Esco Labculture Class II, Type A2 biological safety cabinet meets or exceeds NSF 49 and EN12469:2000 requirements for safety and performance. Each ergonomic unit is factory tested and documented with a commissioning report. Unit features...

Photoswitchable, photoactivatable fluorescent proteins.(August's Innovations)
August 1, 2006... Three fluorescent proteins are offered for in vivo analysis of cellular events. PS-CFP2 is a photo-switchable monomeric fluorescent protein that converts from a cyan to a green fluorescent form when activated by UV-violet light. Highly stable...

Small Animal in vivo labeling.(August's Innovations)
August 1, 2006... SAIVI Small Animal Antibody/Protein labeling kits offer a controlled degree of labeling with Alexa Fluor dyes for in vivo imaging. Bright, photostable Alexa Fluor dyes optimize labeling and purification, and a modulating reagent optimizes...

Benchtop molecular printing system.(August's Innovations)
August 1, 2006... The NanoArrayer benchtop ultramicro- and nanoscale fluid delivery system is a multifaceted platform capable of delivering femtoliter to attoliter volumes of solutions containing biomolecules and other materials to defined locations on surfaces...

Protein Purification Kits.(Proteomics Focus)
August 1, 2006... PrepEase Protein Purification Kits purify recombinant polyhistidine-tagged (His-tagged) recombinant proteins expressed in E. coli using immobilized metal ion affinity chromatography (IMAC). The kits can also purify His-tagged proteins from...

Bacterial Protein Extraction.(Proteomics Focus)
August 1, 2006... SoluLyse non-ionic detergent provides efficient bacterial cell lysis and protein extraction under non-denaturing conditions. Cell walls are perforated without protein being denatured. Soluble proteins remain soluble and are ready to be fully...

Quantify protein expression.(Proteomics Focus)
August 1, 2006... iTRAQ reagents facilitate discovery and quantification of protein expression changes by mass spectrometry across multiple samples. Researchers can simultaneously compare multiple samples; quantify, in absolute terms, specific proteins of...

Fluorescent Protein Stain.(Proteomics Focus)
August 1, 2006... Krypton Protein Stain fluorescent technology provides sensitivity while minimizing quantitation problems associated with differential protein staining. The product detects down to 2 ng protein in SDS-PAGE and 2-D gels in 30 minutes using the...

Simultaneous insertion of 2, 3, or 4 DNA fragments into a single vector.(Application Note)
August 1, 2006... MultiSite Gateway Pro (Invitrogen Corp., Carlsbad, CA) facilitates recombination of up to 4 fragments into a single vector. The method is based on the use of 6 novel DONR vectors and a total of 5 different specific Gateway recombination sites....

Gel Shift Assay.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... ProSHIFT-Gel Shift Assays are EMSA (Electrophoretic Mobility Shift Assay) that take the Gel Shift concept to a level of greater accuracy and specificity using purified whole proteins vs the previous cell extract interaction assays. The known...

FT-IR, Raman Spectral Library Catalog.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... FT-IR and Raman Spectral Libraries Catalog details large and comprehensive collections of compounds including the most common chemicals found in the Aldrich Handbook of Fine Chemicals and the Sigma Biochemical Condensed Phase Library, and is...

Microarray analysis of archived FFPE clinical samples.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... An improved method for isolating RNA from Formalin-Fixed Paraffin-Embedded (FFPE) samples of sufficient quality and quantity for microarray-based gene expression analysis is available. The method increases percent positive calls on high-density...

Variable amino acid mixtures.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Maintaining recombinant plasmids in yeast or certifying the purity of yeast mutant strains often requires culture media lacking amino acids. Easy access to a variety of quality pre-made amino acid dropout mixtures simplifies the process of...

High yield in vitro transcription.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... AmpliScribe T7-Flash transcription kit produces 160 [micro]g to 180 [micro]g of RNA from 1 [micro]g of DNA template in a standard 30-min reaction. High yields of full-length transcripts are readily obtained from both long and short DNA...

DNA-Free Taq Polymerase, a thermostable enzyme.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... DFS (DNA-Free Sensitive)-Taq Polymerase, a thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus aquaticus strain YT-1, catalyzes the polymerization of nucleotides into duplex DNA in the 5' 3' direction in the presence...

High yield RNA Amplification.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... With just 50 ng of input, MessageAmp II Biotin Enhanced Single Round aRNA Amplification Kit yields enough biotin labeled aRNA for GeneChip analysis, aRNA from the kit results in consistently high Present calls from a wide range of inputs. ...

Web-based DNA array design.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Version 3.5 of eArray Web-based DNA microarray design tool allows collaborators to share information securely. In addition to existing gene expression content, annotation information and probe sequences, the tool provides access to the array...

Human protein extract collection.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Comprehensive collection of ready to-use human protein extracts consists of tissue lysates for use in a range of applications and protocols. Matched human tumor and normal tissue protein extracts accelerate cancer research by providing access...

Prepacked columns.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... HiTrap MabSelect, HiTrap MabSelect Xtra, and HiTrap MabSelect SuRe are prepacked columns for purification of monoclonal antibodies. Designed for lab-scale use, screening of purification conditions, and process development, these 1-ml and 5-ml...

Generate SUMOylated proteins.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... SUMOlink Kits provide a simple method for generating SUMOylated proteins in vitro (SUMO is small ubiquitin related modifier). Using the SUMOylation enzyme cascade, covalent linkage of the carboxy-terminal SUMO-1, -2 or -3 peptide substrates to...

PCR screening System.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... The FlashGel System screens PCR or restriction fragments. DNA fragments of 50 to 4 kb are separated in just five minutes. Less than 1 ng DNA per band is detected. Enclosed, disposable, precast cassettes contain ready-to-use agarose gels and...

MS purified peptides.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... The utilization of LC/MS purification technology in the Sigma-Genosys peptide manufacturing process has significantly increased throughput. In addition, analytical capabilities have been augmented by the introduction of MS/MS detection. This...

Cell Surface Antibodies.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Express Cell Surface Antibodies (Guava Technologies, Inc.) identify subsets of hematopoeric-derived cells by their patterns of protein expression. The antibodies allow examination of very specific population of hematopoetic-derived (blood) cell...

High throughput SNP genotyping services.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Genizon BioSciences is offering Single Nucleotide Polymorphism (SNP) genotyping services. In addition, Genizon offers statistical analysis services using its automated genetic computational pipeline. All services are performed at the company's...

Whole Genome Amplification and Labeling.(Biochemicals & Kits: PRODUCTS)
August 1, 2006... Genome-pULSe arrayCGH Whole Genome Amplification and Labeling Kit combines QIAGEN's REPLI-g kit whole genome amplification (WGA) technology, which allows amplification of very small amounts of genomic DNA isolated from tissue or cells, with...

Automated immunogold labeling system.(APPLICATIONS IN Life Science)
August 1, 2006... The automated EM IGL immunogold labeling system addresses the needs of researchers looking to localize a range of cellular molecules. It eliminates the need for tedious manual labeling of grids and improves reproducibility of results. Reagents...

Compact chiro-optical spectrometer.(APPLICATIONS IN Life Science)
August 1, 2006... The J-815 chiro-optical spectrometer features a compact design and a wavelength range of 163 to 900 nm. With an optional 21 CFR Part 11 compliant package, Spectra Manager II software control is standard for instrument control and data analysis....

Advancement in real-time in vivo and in vitro nitric oxide measurements.(APPLICATIONS IN Life Science)
August 1, 2006... Nitric oxide (NO) was named molecule of the year by Science Magazine in 1992, and its discoverers were awarded the Nobel Prize in 1998. Since that time the recognition of its importance has grown substantially as many of its physiological and...

Automated, robotic liquid handling workstations.(PRODUCTS)
August 1, 2006... The Sciclone family of liquid handling workstations offers fast, reliable and scalable automation solutions for drug discovery applications. The Sciclone i1000 features multi-MEMS technology to provide accurate, independent control of 96...

C[O.sub.2] incubator for high volume cell culture.(PRODUCTS)
August 1, 2006... The ergonomic Reach-In CO2 incubator provides a suitable environment for small-scale, high-volume cell culture. Infrared CO2, temperature, RH and CO2 sensors provide control and uniformity. The incubator provides 30 cu. ft. of culturing space...

Centrifugal evaporators include methods for drying aqueous samples.(PRODUCTS)
August 1, 2006... The miVac centrifugal evaporator is suited for labs that use large numbers of membranes per year. The sample concentration technique ensures complete sample recovery, even when taking proteins to very high concentrations. In most biological...

Automated compound tube and plate replication.(PRODUCTS)
August 1, 2006... The reconfigured BioCel sample-processing platform automates compound tube and plate replication procedures. The compound management configuration handles compounds stored in a variety of formats, including 96-well tube racks and 96-well,...

Desktop-portable image analysis system.(PRODUCTS)
August 1, 2006... The LAS-3000mini CCD analyzes chemiluminescence and bioluminescence, specifically in Western blotting applications. The Super CCD chip enables a resolution of 3.2- to 6.3-million pixels. The F0.85 FUJINON high-sensitivity lens captures faint...

Bulk reagent dispenser employs built-in tracking system.(PRODUCTS)
August 1, 2006... The Multidrop Combi SMART bulk reagent dispenser employs a built-in tracking system that shows the exact lifetime status of the dispensing cassette. This feature provides improved QC and traceability, as well as instantaneous cassette type...

Protein blotting sandwiches for high throughput laboratories.(PRODUCTS)
August 1, 2006... Immobilon-P blotting sandwiches are precut to match commonly used precast electrophoresis gels. They also save time for high throughput labs using chemiluminescent or chromogenic detection methods. Each consists of one sheet of Immobilon-P...

High-sensitivity microarray slides.(PRODUCTS)
August 1, 2006... The AmpliSlide range of high-sensitivity reflective microarray slides combines a proprietary, fluorescence-amplifying dielectric coating with highly efficient binding chemistries. AmpliSlide 570/670 A (aminosilane) is suited for double color...

Module converts microscopes into imaging and spectroscopy platforms.(PRODUCTS)
August 1, 2006... The ConfoCor 3 detection module converts LSM 510 and LSM 510 META microscopes into fully integrated imaging and spectroscopy platforms. The module provides sensitive imaging in fluorescence correlation spectroscopy and other fluctuation...

Digital microscope camera.(CELL BASED Assays SUPPLEMENT)
August 1, 2006... The DP71 microscope digital camera is a 12.5 megapixel, 12-bit digital color camera that displays the native CCD's full-resolution live image at 15 frames per second. It can capture a 12.5 megapixel image with color fidelity, clarity, and an...

High-content reader.(CELL BASED Assays SUPPLEMENT)
August 1, 2006... The cellWoRx High Content Cell Analysis System, an automated fluorescence imaging reader, provides microscopic images and data with a large field of view and high signal-to-noise ratios. Image analysis software based on validated algorithms is...

Multiplexing calcium mobilization and membrane potential assays.(CELL BASED Assays SUPPLEMENT)
August 1, 2006... Abstract We present a multiplexing method for HTS screening: loading the same cells using both the UV dye Fura-2 AM and No Wash Membrane Potential Dye. Multiplexing assays measure orthogonal cell responses (calcium mobilization and...

Ubiquitin Assay Reagents.(CELL BASED ASSAYS SUPPLEMENT)
August 1, 2006... LanthaScreen Ubiquitin Assay Reagents provide high-throughput screening to either monitor the rate of ubiquitination of target proteins or to measure the extent of target protein ubiquitination. By incorporating the TR-FRET donor, terbium (Tb),...

Chemiluminescent PARG assay.(CELL BASED ASSAYS SUPPLEMENT)
August 1, 2006... This 96-well PARG assay kit measures the loss of biotinylated PAR attached to strip wells in a 96-well format. (Poly(ADP-ribose) glycohydrolase (PARG) degrades poly(ADP-ribose) (PAR) polymers synthesized by poly(ADP-ribose) polymerase (PARP1).)...

cAMP assays.(CELL BASED ASSAYS SUPPLEMENT)
August 1, 2006... Three cyclic AMP (cAMP) assays incorporate d2, a small molecule organic dye that offers assay stability when measuring G-Protein Coupled Receptor (GPCR) activation. The cAMP dynamic 2 and cAMP femto 2 assays, along with the cAMP HiRange assay...

Human neural stem cell functional identification kit.(CELL BASED ASSAYS SUPPLEMENT)
August 1, 2006... Human neural stem cell (NSC) functional identification kit contains media supplements that can be used for the short-term maintenance, differentiation and expansion of NSCs, and for their induction into astrocyte, neuron and oligodendrocyte...

Biological pathways resource.(CELL BASED ASSAYS SUPPLEMENT)
August 1, 2006... PathFinder presents biological signaling and metabolic pathways in an interactive, graphical representation of the cell that can be used to explore the relationships between different pathway components. The online tool provides links...

Safety Cabinet.(Safety Focus)
August 1, 2006... Purifier Digital Delta Series Safety Cabinets provide personnel, sample and environmental protection and are suitable for work with agents that require Biosafety Level 1, 2 or 3 containment. A Smart-Start System allows programming of start-up...

Exhaust enclosures.(Safety Focus)
August 1, 2006... AirClean Series 200 ductless enclosures can be used as a stand-alone lab fixture, and are particularly suited for use with all Buchi Rotary Evaporators. The units are quiet during operation ( Brinkmann Instruments, Inc. 800-645-3050 ...

Gel extraction tool.(Safety Focus)
August 1, 2006... The x-tracta disposable agarose gel tool neatly cuts and picks up uniform gel slices, eliminating possible cross-contamination from razor blades, tweezers, or other gel cutting methods. The process reduces the time that researchers and DNA...

Radiation protection pipette shields.(Safety Focus)
August 1, 2006... These shields fit snugly on the shaft of the Gilson or BioHit pipettes for which they were designed. They protect the hand from radiation and enable safe dispensing into small and narrow neck containers. Beta emissions of [sup.32]P, [sup.35]S,...

UV micro cuvette.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... Designed for the photometric analysis of nucleic acids and proteins, this UV transparent cuvette is suitable for measurements in the visible and ultraviolet range down to 220 nm. It is manufactured from transparent plastic. Two versions are...

Stirring hot plate.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... EchoTherm Model HS40 is programmed through a front panel membrane switch and LCD. The unit can store 10 programs in memory, each up to 10 steps each, where each step is a temperature, temperature ramp rate, stirring speed and time. Each program...

Single-tab aluminum foils.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... These sealing foils are classic single-tab-design adhesive sealing foils for protecting samples in microplates during PCR gene amplification or cold storage. AlumaSeal 96 foils are designed to fit easily inside the rim of raised-rim plates....

Fiber optic illuminator.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... The DC950 150-watt quartz halogen regulated illuminator features DC regulated output, fast lamp response, and a 0-5 VDC remote intensity control interface with linear voltage adjustment (8 bit D/A module available). It can be operated remotely...

Cryogenic vials, accessories.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... Cryos cryogenic storage vials provide safe storage of samples and specimens at sub-zero temperatures. Round bottom, conical or self-standing styles are made in 1 to 5 cc capacities. Packaged sterile with internal or external screw caps, with...

TOC Monitor.(Apparatus & Consumables: PRODUCTS)
August 1, 2006... A10 Total Oxidizable Carbon (TOC) Monitor measures from 1 to 999 ppb of organic substances in pure and ultrapure water at a rate of up to 200 L/hr. The monitor is shipped with a certificate of calibration and is designed according to the...

Automate compound weighing, preparation for library stores.(Compound Library Management Focus)
August 1, 2006... MOSS Modular Sample Preparation System is designed to bridge the gap between stores of hundreds of thousands of library compounds and high throughput screening laboratories. The system combines weighing technology and software to automate assay...

Automated cell culture system.(Compound Library Management Focus)
August 1, 2006... Automated CompacT SelecT cell culture system manages the process of preparing, culturing and harvesting cells in T-flasks and delivering them into assay-ready plates. Continuous unattended operation provides simultaneous culture of multiple and...

Dispense 650,000 different compounds in 24 hours.(Compound Library Management Focus)
August 1, 2006... Echo 555 dispenses more than 650,000 different compounds in 24 hours. An acoustic droplet ejection system transfers liquids without contact. Direct plate-to-plate transfers is performed on droplets down to 2.5 nl. Applications include plate...

High throughput liquid transfer, reagent addition.(Compound Library Management Focus)
August 1, 2006... The 96-channel Platemaker provides both high-throughput liquid transfers and reagent additions on one integrated platform. Maximum throughput is offered for HTS and genomic applications. Independent 96-tip and 8-tip heads can access plates of...

UPLC column hardware.(Instruments & Systems: PRODUCTS)(Column)
August 1, 2006... IsoBar column hardware, designed for use in Ultra-Performance Liquid Chromatography (UPLC). All-metal construction combines with high strength threaded end fittings to enable operation at pressures up to 50,000 psi and make it ideal for ...

Electrochemical detection system software.(Instruments & Systems: PRODUCTS)
August 1, 2006... CoulArray application software V 3.0 (ESA Biosciences) provides effective operation of the CoulArray HPLC electrochemical array detector. The software features single window interface operating controls and enables the detector to effectively...

Multi-angle light scattering detector.(Instruments & Systems: PRODUCTS)
August 1, 2006... The miniDAWN TREOS multi-angle light-scattering (MALS) detector (Wyatt Technology) provides measurement of multi-angle light scattering (MALS) for small molecules. It has over twice the signal to noise ratio and 100x the dynamic range of the...

Stereo microscope series.(Instruments & Systems: PRODUCTS)
August 1, 2006... SZX2 series microscopes offer a 16.4:1 zoom ratio and high resolution. Model SZX16 offer high numerical aperture objectives, including a 2x objective with an NA of 0.3, producing a resolution of 900 line pairs per millimeter. The objectives...

Nanopositioning controller.(Instruments & Systems: PRODUCTS)
August 1, 2006... The E-761 PCI-bus digital controller for closed-loop piezoelectric positioners and scanners controls 3 logical axes of closed-loop piezo nanopositioning systems with capacitive position feedback. The PCI interface provides high throughput and...

Data acquisition board.(Instruments & Systems: PRODUCTS)
August 1, 2006... DAP 5000a/526 includes 16 analog inputs, 2 analog outputs, 16 digital inputs, and 16 digital outputs. External rack-mounted hardware extends these channel counts to 512, 66, 128, and 1024 respectively. The board acquires 14-bit data at up to...

Pathway analysis software.(Instruments & Systems: PRODUCTS)
August 1, 2006... IPA 4.0 enables advanced search, reporting, path explorer and supports Mac platforms. Users will be able to conduct and save advanced searches for genes, proteins or biological relationships, create reports on bibliographies of pathway...

Microscope with MiniVID camera.(Instruments & Systems: PRODUCTS)
August 1, 2006... The Revelation III Microscope and MiniVID camera offer 10X/18 wide-field eyepieces and a quadruple forward-facing nosepiece. The optics, DIN Achromatic or Semi-Plan, with 4x, 10x, 40x and 100x off objectives provide affordable quality. The...

UPLC applications.(Instruments & Systems: PRODUCTS)
August 1, 2006... Ultra Performance LC (UPLC) technology products for bioseparations from Waters Corp. include UPLC amino acid and peptide analysis application solutions. Both system solutions come with chemistry, instrumentation, methodology, documentation and...

Visual analytics environment.(Instruments & Systems: PRODUCTS)
August 1, 2006... DecisionSite visual analytics application enables life scientists to deploy statistical tools written in SAS, Insightful S-PLUS, and R Open Source directly within the application. Various statistical functions are built-in. Computational...

Seeing further with the "up-and-down" microscope.(Best Practices)
August 1, 2006... Kenneth N. Fish needed a new microscope. The problem was, he needed one that didn't exist. Dr. Fish, an assistant professor of psychiatry at the University of Pittsburgh, studies long-term memory by focusing on molecular signaling among...